Review





Similar Products

mif inhibitors  (MedChemExpress)
95
MedChemExpress mif inhibitors
Mif Inhibitors, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mif inhibitors/product/MedChemExpress
Average 95 stars, based on 1 article reviews
mif inhibitors - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
mif inhibitor  (MedChemExpress)
94
MedChemExpress mif inhibitor
a The expressions of TLR4, MyD88, TRAF6, p-p65, p65, p-IκBαand IκBαin the midbrain of mice were detected by Western blotting. b –f Quantitative statistical analysis of the band density of TLR4, MyD88, TRAF6, p-p65/p65 and p-iκBα/IκBα, respectively. n = 3 per group in animal experiments. g Using <t>specific</t> <t>inhibitor</t> ISO-1 or <t>MIF-siRNA,</t> downregulation of MIF inhibits TLR4 receptor activation, thereby inhibiting the MyD88-dependent pathway-mediated pathway downstream of TLR4 and reducing nuclear translocation of the transcription factor NF-κBp65, thereby reducing pro-inflammatory factors (IL-1β, IL-6, TNF-α) transcriptional upregulation to alleviate neuroinflammation and ultimately protect dopaminergic nerves from microglia activation-induced neurological damage and degeneration. All data are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA (NS: not statistically significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Mif Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mif inhibitor/product/MedChemExpress
Average 94 stars, based on 1 article reviews
mif inhibitor - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
mif inhibitor 4 iodo 6phenylpyrimidine  (MedChemExpress)
94
MedChemExpress mif inhibitor 4 iodo 6phenylpyrimidine
a The expressions of TLR4, MyD88, TRAF6, p-p65, p65, p-IκBαand IκBαin the midbrain of mice were detected by Western blotting. b –f Quantitative statistical analysis of the band density of TLR4, MyD88, TRAF6, p-p65/p65 and p-iκBα/IκBα, respectively. n = 3 per group in animal experiments. g Using <t>specific</t> <t>inhibitor</t> ISO-1 or <t>MIF-siRNA,</t> downregulation of MIF inhibits TLR4 receptor activation, thereby inhibiting the MyD88-dependent pathway-mediated pathway downstream of TLR4 and reducing nuclear translocation of the transcription factor NF-κBp65, thereby reducing pro-inflammatory factors (IL-1β, IL-6, TNF-α) transcriptional upregulation to alleviate neuroinflammation and ultimately protect dopaminergic nerves from microglia activation-induced neurological damage and degeneration. All data are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA (NS: not statistically significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Mif Inhibitor 4 Iodo 6phenylpyrimidine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mif inhibitor 4 iodo 6phenylpyrimidine/product/MedChemExpress
Average 94 stars, based on 1 article reviews
mif inhibitor 4 iodo 6phenylpyrimidine - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
mif inhibitor 4 iodo 6 phenylpyrimidine  (MedChemExpress)
94
MedChemExpress mif inhibitor 4 iodo 6 phenylpyrimidine
a The expressions of TLR4, MyD88, TRAF6, p-p65, p65, p-IκBαand IκBαin the midbrain of mice were detected by Western blotting. b –f Quantitative statistical analysis of the band density of TLR4, MyD88, TRAF6, p-p65/p65 and p-iκBα/IκBα, respectively. n = 3 per group in animal experiments. g Using <t>specific</t> <t>inhibitor</t> ISO-1 or <t>MIF-siRNA,</t> downregulation of MIF inhibits TLR4 receptor activation, thereby inhibiting the MyD88-dependent pathway-mediated pathway downstream of TLR4 and reducing nuclear translocation of the transcription factor NF-κBp65, thereby reducing pro-inflammatory factors (IL-1β, IL-6, TNF-α) transcriptional upregulation to alleviate neuroinflammation and ultimately protect dopaminergic nerves from microglia activation-induced neurological damage and degeneration. All data are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA (NS: not statistically significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Mif Inhibitor 4 Iodo 6 Phenylpyrimidine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mif inhibitor 4 iodo 6 phenylpyrimidine/product/MedChemExpress
Average 94 stars, based on 1 article reviews
mif inhibitor 4 iodo 6 phenylpyrimidine - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
mif inhibitors  (Chembridge)
90
Chembridge mif inhibitors
a The expressions of TLR4, MyD88, TRAF6, p-p65, p65, p-IκBαand IκBαin the midbrain of mice were detected by Western blotting. b –f Quantitative statistical analysis of the band density of TLR4, MyD88, TRAF6, p-p65/p65 and p-iκBα/IκBα, respectively. n = 3 per group in animal experiments. g Using <t>specific</t> <t>inhibitor</t> ISO-1 or <t>MIF-siRNA,</t> downregulation of MIF inhibits TLR4 receptor activation, thereby inhibiting the MyD88-dependent pathway-mediated pathway downstream of TLR4 and reducing nuclear translocation of the transcription factor NF-κBp65, thereby reducing pro-inflammatory factors (IL-1β, IL-6, TNF-α) transcriptional upregulation to alleviate neuroinflammation and ultimately protect dopaminergic nerves from microglia activation-induced neurological damage and degeneration. All data are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA (NS: not statistically significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
Mif Inhibitors, supplied by Chembridge, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mif inhibitors/product/Chembridge
Average 90 stars, based on 1 article reviews
mif inhibitors - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
mifepristone mif  (Selleck Chemicals)
93
Selleck Chemicals mifepristone mif
Beclin-1-mediated autophagy was associated with increased differentiation and mineralization of osteoblasts. (A) Cell viability of osteoblasts assayed by CCK-8 method. (B) Osteoblasts stained by alkaline phosphatase (ALP) staining to observe osteogenic differentiation. (C) Osteoblasts stained by Alizarin Red S (ARS) to observe bone mineralized nodules. (D) Immunoblotting assay to detect the expression of osteogenesis-related proteins (OPG and RUNX2), in the osteoblasts. Relative protein expression of OPG (E) and RUNX2 (F) in the indicated groups. MUT, osteoblasts cells with Beclin-1 overexpression; Bafilomycin A1 (Baf), autophagy inhibitor; <t>Mifepristone</t> (Mif), autophagy activator; Rapamycin (Rapa), autophagy activator. ****p < 0.0001.
Mifepristone Mif, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mifepristone mif/product/Selleck Chemicals
Average 93 stars, based on 1 article reviews
mifepristone mif - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
4-ipp  (MedChemExpress)
94
MedChemExpress 4-ipp
Beclin-1-mediated autophagy was associated with increased differentiation and mineralization of osteoblasts. (A) Cell viability of osteoblasts assayed by CCK-8 method. (B) Osteoblasts stained by alkaline phosphatase (ALP) staining to observe osteogenic differentiation. (C) Osteoblasts stained by Alizarin Red S (ARS) to observe bone mineralized nodules. (D) Immunoblotting assay to detect the expression of osteogenesis-related proteins (OPG and RUNX2), in the osteoblasts. Relative protein expression of OPG (E) and RUNX2 (F) in the indicated groups. MUT, osteoblasts cells with Beclin-1 overexpression; Bafilomycin A1 (Baf), autophagy inhibitor; <t>Mifepristone</t> (Mif), autophagy activator; Rapamycin (Rapa), autophagy activator. ****p < 0.0001.
4 Ipp, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4-ipp/product/MedChemExpress
Average 94 stars, based on 1 article reviews
4-ipp - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
mif inhibitor iso 1  (MedChemExpress)
95
MedChemExpress mif inhibitor iso 1
Beclin-1-mediated autophagy was associated with increased differentiation and mineralization of osteoblasts. (A) Cell viability of osteoblasts assayed by CCK-8 method. (B) Osteoblasts stained by alkaline phosphatase (ALP) staining to observe osteogenic differentiation. (C) Osteoblasts stained by Alizarin Red S (ARS) to observe bone mineralized nodules. (D) Immunoblotting assay to detect the expression of osteogenesis-related proteins (OPG and RUNX2), in the osteoblasts. Relative protein expression of OPG (E) and RUNX2 (F) in the indicated groups. MUT, osteoblasts cells with Beclin-1 overexpression; Bafilomycin A1 (Baf), autophagy inhibitor; <t>Mifepristone</t> (Mif), autophagy activator; Rapamycin (Rapa), autophagy activator. ****p < 0.0001.
Mif Inhibitor Iso 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mif inhibitor iso 1/product/MedChemExpress
Average 95 stars, based on 1 article reviews
mif inhibitor iso 1 - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier

Image Search Results


a The expressions of TLR4, MyD88, TRAF6, p-p65, p65, p-IκBαand IκBαin the midbrain of mice were detected by Western blotting. b –f Quantitative statistical analysis of the band density of TLR4, MyD88, TRAF6, p-p65/p65 and p-iκBα/IκBα, respectively. n = 3 per group in animal experiments. g Using specific inhibitor ISO-1 or MIF-siRNA, downregulation of MIF inhibits TLR4 receptor activation, thereby inhibiting the MyD88-dependent pathway-mediated pathway downstream of TLR4 and reducing nuclear translocation of the transcription factor NF-κBp65, thereby reducing pro-inflammatory factors (IL-1β, IL-6, TNF-α) transcriptional upregulation to alleviate neuroinflammation and ultimately protect dopaminergic nerves from microglia activation-induced neurological damage and degeneration. All data are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA (NS: not statistically significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Journal: Communications Biology

Article Title: MIF downregulation attenuates neuroinflammation via TLR4/MyD88/TRAF6/NF-κB pathway to protect dopaminergic neurons in Parkinson’s disease model

doi: 10.1038/s42003-025-08997-7

Figure Lengend Snippet: a The expressions of TLR4, MyD88, TRAF6, p-p65, p65, p-IκBαand IκBαin the midbrain of mice were detected by Western blotting. b –f Quantitative statistical analysis of the band density of TLR4, MyD88, TRAF6, p-p65/p65 and p-iκBα/IκBα, respectively. n = 3 per group in animal experiments. g Using specific inhibitor ISO-1 or MIF-siRNA, downregulation of MIF inhibits TLR4 receptor activation, thereby inhibiting the MyD88-dependent pathway-mediated pathway downstream of TLR4 and reducing nuclear translocation of the transcription factor NF-κBp65, thereby reducing pro-inflammatory factors (IL-1β, IL-6, TNF-α) transcriptional upregulation to alleviate neuroinflammation and ultimately protect dopaminergic nerves from microglia activation-induced neurological damage and degeneration. All data are presented as mean ± SEM. Statistical analysis was performed using one-way ANOVA (NS: not statistically significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: MIF inhibitor, (S, R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester (ISO-1, HY-16692) and Resatorvid (Toll-like receptor 4 inhibitor, HY-11109) were purchased from MedChemExpress.

Techniques: Western Blot, Activation Assay, Translocation Assay

Beclin-1-mediated autophagy was associated with increased differentiation and mineralization of osteoblasts. (A) Cell viability of osteoblasts assayed by CCK-8 method. (B) Osteoblasts stained by alkaline phosphatase (ALP) staining to observe osteogenic differentiation. (C) Osteoblasts stained by Alizarin Red S (ARS) to observe bone mineralized nodules. (D) Immunoblotting assay to detect the expression of osteogenesis-related proteins (OPG and RUNX2), in the osteoblasts. Relative protein expression of OPG (E) and RUNX2 (F) in the indicated groups. MUT, osteoblasts cells with Beclin-1 overexpression; Bafilomycin A1 (Baf), autophagy inhibitor; Mifepristone (Mif), autophagy activator; Rapamycin (Rapa), autophagy activator. ****p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Efficacy of the Bushen Jianpi Huoxue Formula on Beclin-1/Bcl-2-mediated autophagy and apoptosis in osteoblasts

doi: 10.3389/fphar.2024.1513298

Figure Lengend Snippet: Beclin-1-mediated autophagy was associated with increased differentiation and mineralization of osteoblasts. (A) Cell viability of osteoblasts assayed by CCK-8 method. (B) Osteoblasts stained by alkaline phosphatase (ALP) staining to observe osteogenic differentiation. (C) Osteoblasts stained by Alizarin Red S (ARS) to observe bone mineralized nodules. (D) Immunoblotting assay to detect the expression of osteogenesis-related proteins (OPG and RUNX2), in the osteoblasts. Relative protein expression of OPG (E) and RUNX2 (F) in the indicated groups. MUT, osteoblasts cells with Beclin-1 overexpression; Bafilomycin A1 (Baf), autophagy inhibitor; Mifepristone (Mif), autophagy activator; Rapamycin (Rapa), autophagy activator. ****p < 0.0001.

Article Snippet: Mifepristone (Mif) (Selleck Chemicals) was utilized to inhibit the binding of Beclin-1 to Bcl-2.

Techniques: CCK-8 Assay, Staining, Western Blot, Expressing, Over Expression

The Bushen Jianpi Huoxue Formula inhibited cellular autophagy while was not through promoting the binding of Beclin-1 and Bcl-2. (A) CCK-8 assay detected the cell viability of osteoblasts; (B) Autophagic vacuoles labelled with MDC; (C) MDC-labelled cells detected by flow cytometry; (D) Quantification of autophagy rate; (E) Lysosomes stained by LysoTracker probe; (F) The binding of Beclin-1 to Bcl-2 detected by immunoprecipitation. (G) Quantification of Beclin-1 and Bcl-2 complex. MUT, osteoblasts cells with Beclin-1 overexpression; Mifepristone (Mif), autophagy activator. ns p > 0.05; ****p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Efficacy of the Bushen Jianpi Huoxue Formula on Beclin-1/Bcl-2-mediated autophagy and apoptosis in osteoblasts

doi: 10.3389/fphar.2024.1513298

Figure Lengend Snippet: The Bushen Jianpi Huoxue Formula inhibited cellular autophagy while was not through promoting the binding of Beclin-1 and Bcl-2. (A) CCK-8 assay detected the cell viability of osteoblasts; (B) Autophagic vacuoles labelled with MDC; (C) MDC-labelled cells detected by flow cytometry; (D) Quantification of autophagy rate; (E) Lysosomes stained by LysoTracker probe; (F) The binding of Beclin-1 to Bcl-2 detected by immunoprecipitation. (G) Quantification of Beclin-1 and Bcl-2 complex. MUT, osteoblasts cells with Beclin-1 overexpression; Mifepristone (Mif), autophagy activator. ns p > 0.05; ****p < 0.0001.

Article Snippet: Mifepristone (Mif) (Selleck Chemicals) was utilized to inhibit the binding of Beclin-1 to Bcl-2.

Techniques: Binding Assay, CCK-8 Assay, Flow Cytometry, Staining, Immunoprecipitation, Over Expression

Effects of the Bushen Jianpi Huoxue Formula on apoptosis of osteoblasts. (A) Apoptotic cells were marked by Annexin V-PE/7-AAD and observed by flow cytometry. (B) Quantitative analysis of apoptosis rate of osteoblasts detected by flow cytometry. (C) Hoechest staining was performed to observe apoptotic cells. (D) Immunoblotting assay to detect the expression of apoptotic proteins (Bax, Bcl-2, Bax/Bcl-2, Caspase-3, and Caspase-9) in osteoblasts. Relative expression of Bax (E) , Bcl-2 (F) , Bax (G) , Caspase-3 (H) , and Caspase-9 (I) was quantitatively presented based on immunoblotting assay. MUT, osteoblasts cells with Beclin-1 overexpression; Mifepristone (Mif), autophagy activator. ns p > 0.05, **p < 0.01, ****p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Efficacy of the Bushen Jianpi Huoxue Formula on Beclin-1/Bcl-2-mediated autophagy and apoptosis in osteoblasts

doi: 10.3389/fphar.2024.1513298

Figure Lengend Snippet: Effects of the Bushen Jianpi Huoxue Formula on apoptosis of osteoblasts. (A) Apoptotic cells were marked by Annexin V-PE/7-AAD and observed by flow cytometry. (B) Quantitative analysis of apoptosis rate of osteoblasts detected by flow cytometry. (C) Hoechest staining was performed to observe apoptotic cells. (D) Immunoblotting assay to detect the expression of apoptotic proteins (Bax, Bcl-2, Bax/Bcl-2, Caspase-3, and Caspase-9) in osteoblasts. Relative expression of Bax (E) , Bcl-2 (F) , Bax (G) , Caspase-3 (H) , and Caspase-9 (I) was quantitatively presented based on immunoblotting assay. MUT, osteoblasts cells with Beclin-1 overexpression; Mifepristone (Mif), autophagy activator. ns p > 0.05, **p < 0.01, ****p < 0.0001.

Article Snippet: Mifepristone (Mif) (Selleck Chemicals) was utilized to inhibit the binding of Beclin-1 to Bcl-2.

Techniques: Flow Cytometry, Staining, Western Blot, Expressing, Over Expression

Effects of the intervention of the formula of Bushen Jianpi Huoxue Formula on the differentiation and mineralization of osteoblasts. (A) ALP staining was used to observe the mineralized nodules of osteoblasts. (B) Alizarin red staining was used to observe the mineralized nodules of bones. (C) Osteogenesis-related proteins (OPG and RUNX2) in osteoblasts detected by immunoblotting assay. Relative expression of OPG (D) and RUNX2 (E) in osteoblasts of the indicated groups. MUT, osteoblasts cells with Beclin-1 overexpression; Mifepristone (Mif), autophagy activator. ****p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Efficacy of the Bushen Jianpi Huoxue Formula on Beclin-1/Bcl-2-mediated autophagy and apoptosis in osteoblasts

doi: 10.3389/fphar.2024.1513298

Figure Lengend Snippet: Effects of the intervention of the formula of Bushen Jianpi Huoxue Formula on the differentiation and mineralization of osteoblasts. (A) ALP staining was used to observe the mineralized nodules of osteoblasts. (B) Alizarin red staining was used to observe the mineralized nodules of bones. (C) Osteogenesis-related proteins (OPG and RUNX2) in osteoblasts detected by immunoblotting assay. Relative expression of OPG (D) and RUNX2 (E) in osteoblasts of the indicated groups. MUT, osteoblasts cells with Beclin-1 overexpression; Mifepristone (Mif), autophagy activator. ****p < 0.0001.

Article Snippet: Mifepristone (Mif) (Selleck Chemicals) was utilized to inhibit the binding of Beclin-1 to Bcl-2.

Techniques: Staining, Western Blot, Expressing, Over Expression

Effects of the intervention of the formula of Bushen Jianpi Huoxue Formula on the protein expression of endoplasmic reticulum stress-related gene. (A) Immunoblotting assay for detecting protein expression of osteogenesis-related genes in osteoblasts; Relative protein expression of AFT4 (B) , CHOP (C) , eIF2α (D) , GRP78 (E) were quantified according the results from immunoblotting assay. MUT, osteoblasts cells with Beclin-1 overexpression; Mifepristone (Mif), autophagy activator. ****p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Efficacy of the Bushen Jianpi Huoxue Formula on Beclin-1/Bcl-2-mediated autophagy and apoptosis in osteoblasts

doi: 10.3389/fphar.2024.1513298

Figure Lengend Snippet: Effects of the intervention of the formula of Bushen Jianpi Huoxue Formula on the protein expression of endoplasmic reticulum stress-related gene. (A) Immunoblotting assay for detecting protein expression of osteogenesis-related genes in osteoblasts; Relative protein expression of AFT4 (B) , CHOP (C) , eIF2α (D) , GRP78 (E) were quantified according the results from immunoblotting assay. MUT, osteoblasts cells with Beclin-1 overexpression; Mifepristone (Mif), autophagy activator. ****p < 0.0001.

Article Snippet: Mifepristone (Mif) (Selleck Chemicals) was utilized to inhibit the binding of Beclin-1 to Bcl-2.

Techniques: Expressing, Western Blot, Over Expression

Effects of the intervention of the formula of Bushen Jianpi Huoxue Formula on WNT/β-CATENIN pathway. (A) Immunoblotting assay for detecting protein expression of osteogenesis-related genes in osteoblasts; Relative protein expression of ALP (B) , OPN (C) , WNT1 (D) , β-CATENIN (E) were quantified according the results from immunoblotting assay. MUT, osteoblasts cells with Beclin-1 overexpression; Mifepristone (Mif), autophagy activator. p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Efficacy of the Bushen Jianpi Huoxue Formula on Beclin-1/Bcl-2-mediated autophagy and apoptosis in osteoblasts

doi: 10.3389/fphar.2024.1513298

Figure Lengend Snippet: Effects of the intervention of the formula of Bushen Jianpi Huoxue Formula on WNT/β-CATENIN pathway. (A) Immunoblotting assay for detecting protein expression of osteogenesis-related genes in osteoblasts; Relative protein expression of ALP (B) , OPN (C) , WNT1 (D) , β-CATENIN (E) were quantified according the results from immunoblotting assay. MUT, osteoblasts cells with Beclin-1 overexpression; Mifepristone (Mif), autophagy activator. p < 0.0001.

Article Snippet: Mifepristone (Mif) (Selleck Chemicals) was utilized to inhibit the binding of Beclin-1 to Bcl-2.

Techniques: Western Blot, Expressing, Over Expression